OrangerV1, France, Analysis, bibRecord, 000211

Isolation of DNA from the uncultured "Candidatus liberobacter" species associated with citrus huanglongbing by RAPD

Identifieur interne : 000211 ( France/Analysis ); précédent : 000210; suivant : 000212

Isolation of DNA from the uncultured "Candidatus liberobacter" species associated with citrus huanglongbing by RAPD

Auteurs : A. Hocquellet [France] ; J. M. Bove [France] ; M. Garnier [France]

Source :

Current microbiology [ 0343-8651 ] ; 1999.

RBID : Pascal:99-0252802

Descripteurs français

Pascal (Inist)
- Citrus sinensis, Bactériose, DNA, Marqueur RAPD, Caractérisation, Gène, Clonage moléculaire, Méthode, Liberobacter.

English descriptors

KwdEn :
- Bacteriosis, Characterization, Citrus sinensis, DNA, Gene, Method, Molecular cloning, Random amplified polymorphic DNA marker.

Abstract

"Candidatus Liberobacter," the uncultured bacterium associated with citrus Huanglongbing (HLB) disease, is an α-Proteobacteria, and two species, "Candidatus L. africanum' and "Candidatus L. asiaticum," have been characterized by sequence analysis of the 16S rDNA and β operon (rplKAJL-rpoBC) genes. These genes were isolated by PCR and random cloning of DNA from infected plants. However, this strategy is laborious and allowed selection of only three Liberobacter DNA fragments. In this paper, we described isolation of additional genes using Random Amplified Polymorphic DNA (RAPD). In total, 102 random 10-mer primers were used in PCR reactions on healthy and Liberobacter-infected plant DNA. Eight DNA bands amplified from infected plant DNA were cloned and analyzed. Six of them were found to be part of the Liberobacter genome by sequence and hybridization experiments. On these DNA fragments, four genes were identified: nusG, pgm, omp, and a hypothetical protein gene. These results indicate that RAPD can be used to clone DNA of uncultured organisms.

Affiliations:

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Pascal:99-0252802

Le document en format XML

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<front><div type="abstract" xml:lang="en">"Candidatus Liberobacter," the uncultured bacterium associated with citrus Huanglongbing (HLB) disease, is an α-Proteobacteria, and two species, "Candidatus L. africanum' and "Candidatus L. asiaticum," have been characterized by sequence analysis of the 16S rDNA and β operon (rplKAJL-rpoBC) genes. These genes were isolated by PCR and random cloning of DNA from infected plants. However, this strategy is laborious and allowed selection of only three Liberobacter DNA fragments. In this paper, we described isolation of additional genes using Random Amplified Polymorphic DNA (RAPD). In total, 102 random 10-mer primers were used in PCR reactions on healthy and Liberobacter-infected plant DNA. Eight DNA bands amplified from infected plant DNA were cloned and analyzed. Six of them were found to be part of the Liberobacter genome by sequence and hybridization experiments. On these DNA fragments, four genes were identified: nusG, pgm, omp, and a hypothetical protein gene. These results indicate that RAPD can be used to clone DNA of uncultured organisms.</div>
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Isolation of DNA from the uncultured "Candidatus liberobacter" species associated with citrus huanglongbing by RAPD

Isolation of DNA from the uncultured "Candidatus liberobacter" species associated with citrus huanglongbing by RAPD

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri